Pseudomonas aeruginosa amidase: Aggregation in recombinant Escherichia coli
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چکیده
منابع مشابه
Pseudomonas aeruginosa amidase: aggregation in recombinant Escherichia coli.
The effect of cultivation parameters such as temperature incubation, IPTG induction and ethanol shock on the production of Pseudomonas aeruginosa amidase (E.C.3.5.1.4) in a recombinant Escherichia coli strain in LB ampicillin culture medium was investigated. The highest yield of soluble amidase, relatively to other proteins, was obtained in the condition at 37°C using 0.40 mM IPTG to induce gro...
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ژورنال
عنوان ژورنال: Biotechnology Journal
سال: 2011
ISSN: 1860-6768
DOI: 10.1002/biot.201000321